Extract on LPS-Induced Rat Lung Injury

by Vafa Baradaran Rahimi 1,2,†,Hassan Rakhshandeh 1,†,Federica Raucci 3,Benedetta Buono 3,Reza Shirazinia 4,Alireza Samzadeh Kermani 5,Francesco Maione 3,*,Nicola Mascolo 3 andVahid Reza Askari 1,6,*1Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran2Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran3Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy4Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran 1419963111, Iran5Department of Chemistry, Faculty of Science, University of Zabol, Zabol 35856-98613, Iran6Neurogenic Inflammation Research Centre, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran*Authors to whom correspondence should be addressed.These authors share first co-authorship.Molecules201924(1), 139; https://doi.org/10.3390/molecules24010139Received: 16 December 2018 / Revised: 26 December 2018 / Accepted: 26 December 2018 / Published: 1 January 2019(This article belongs to the Special Issue Role of Natural Compounds in Inflammation and Inflammatory-Related Diseases)View Full-TextDownload PDFBrowse FiguresCitation Export

Abstract

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are classified as two lung complications arising from various conditions such as sepsis, trauma, and lung inflammation. Previous studies have shown that the extract of the leaves of Portulaca oleracea (PO) possesses anti-inflammatory and anti-oxidant activities. In the present study, the effects of PO (50–200 mg/kg) and dexamethasone (Dexa; 1.5 mg/kg) on lipopolysaccharide (LPS)-induced ALI were investigated. Subsequentially, the lung wet/dry ratio; white blood cells (WBC); levels of nitric oxide (NO); myeloperoxidase (MPO); malondialdehyde (MDA); thiol groups formation; super oxide dismutase (SOD) and catalase (CAT) activities; and levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, IL-10, prostaglandin E2 (PGE2), and transforming growth factor (TGF)-β in the broncho alveolar lavage fluid (BALF) were evaluated in order to demonstrate the anti-oxidant and anti-inflammatory activity of PO. Our results show that PO suppresses lung inflammation by the reduction of IL-β, IL-6, TNF-α, PGE2, and TGF-β, as well as by the increase of IL-10 levels. We also found that PO improves the level of WBC, MPO, and MDA, as well as thiol group formation and SOD and CAT activities, compared with the LPS group. The results of our investigation also show that PO significantly decreased the lung wet/dry ratio as an index of interstitial edema. Taken together, our findings reveal that PO extract dose-dependently displays anti-oxidant and anti-inflammatory activity against LPS-induced rat ALI, paving the way for rational use of PO as a protective agent against lung-related inflammatory disease. View Full-TextKeywords: acute lung injuryPortulaca oleraceainflammation