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Phyto-metabolite profiling of Coronil, a herbal medicine for COVID-19, its identification by mass-spectroscopy and quality validation on liquid chromatographic platforms

My remark: contains Withania[Ashwagandha], Basilicum [Tulsi] and another less well known Ayurvedic herb [Tinospora cordifolia]

Acharya Balkrishna et al. J Sep Sci. 2021.Show details   Abstract    PubMed    PMID  Cite


Coronil is a tri-herbal medicine consisting of immunomodulatory herbs, Withania somnifera, Tinospora cordifolia, and Ocimum sanctum. The formulation has been developed specifically as a remedy for Corona virus. Current investigation is aimed to identify the phytoconstituents in Coronil utilizing Ultra-performance liquid chromatography-mass spectrometry coupled with quadrapole time of flight and to establish its quality standardization using HPLC and High Performance Thin Layer Chromatography. Out of 52 identified compounds, cordifolioside A, magnoflorine, rosmarinic acid, palmatine, withanoside IV, withanoside V, withanone, betulinic acid and ursolic acid were quantified in fifteen batches of Coronil on validated HPLC method. Similarly, withanoside IV, withaferin A, magnoflorine, palmatine, rosmarinic acid and ursolic acid were analysed on High Performance Thin Layer Chromatography. Methods were validated as per the International Council for Harmonization guidelines. These methods, were specific, reproducible, accurate, precise, linearity (r2 > 0.99) and percent recoveries within the prescribed limits. The content uniformity of Coronil was ascertained using Fourier Transform Infra-Red spectroscopy. Results indicated that, validated methods were fit for its intended use; and the analytical quality of Coronil was consistent across the batches. Taken together, these developed methods could drive the analytical quality control of herbal medicines like Coronil, and other formulations containing similar chemical profiles. This article is protected by copyright. All rights reserved.

Keywords: Coronil; ayurveda; liquid chromatography; mass spectrometry; validation.